Immunoprecipitation and the High Sensitivity Protein 250 Assay
نویسندگان
چکیده
منابع مشابه
Immunoprecipitation and the High Sensitivity Protein 250 Assay Combining Specific and Sensitive Detection of Proteins with the Agilent 2100 Bioanalyzer
A new method for the targeted analysis of proteins is presented that combines the specificity of immunoprecipitation with the sensitivity of protein detection on microchips using the High Sensitivity Protein 250 assay for the Agilent 2100 Bioanalyzer. As as an alternative to Western blotting, this method is valuable for researchers engaged, for example, in protein expression and purification in...
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The Agilent High Sensitivity Protein 250 assay for the Agilent 2100 bioanalyzer analyzes proteins from 10 to 250 kDa. This assay is based on the detection of fluorescently labeled proteins that are separated electrophoretically on microfluidic chips. It provides sizing and quantification of proteins over a large concentration range, including concentrations as low as 1 pg/μL labeled BSA on-chip...
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SDS-polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins according to their molecular weights. For highest sensitivity, gels are commonly silver stained using a laborious procedure with low reproducibility, which yields low reproducibility and insufficient quantification capabilities. In this publication we demonstrate the performance of a newly developed Agilent 2100 bioanalyzer me...
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Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of DNA-binding proteins and the mechanisms by which they control and guide gene regulation pathways and cellular p...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2010
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113404